First report on genetic characterization, cell-surface properties and pathogenicity of Lactococcus garvieae, emerging pathogen isolated from cage-cultured cobia (Rachycentron canadum).

  title={First report on genetic characterization, cell-surface properties and pathogenicity of Lactococcus garvieae, emerging pathogen isolated from cage-cultured cobia (Rachycentron canadum).},
  author={Shreesha Rao and Hieu Trung Pham and Sayuj Poudyal and Li-Wu Cheng and Sandra Celenia Nazareth and Pei-Chi Wang and Shih‐Chu Chen},
  journal={Transboundary and emerging diseases},
The diseased cage-cultured cobia (Rachycentron canadum) displayed clinical signs, haemorrhagic eyes, dorsal darkness and gross pathological lesions, enlargement of spleen and liver. Haemorrhages were found in brain, heart and liver with cumulative mortality rates ranging from 20% to 50%. Extensive congestion in the heart, liver, spleen, kidney, and brain were observed histopathologically. Epicarditis and meningitis were also revealed in diseased cobia. All isolates recovered from the organs… 

First report of acanthocephalan parasite, Longicollum pagrosomi Yamaguti, 1935 in cultured red snapper (Lutjanus erythropterus) in Taiwan.

For the first time, Longicollum pagrosomi Yamaguti, 1935, an acanthocephalan parasite, has been characterized with morphological, histopathological and molecular detail in farmed red snapper

Sarcodia suiae Water Extract Promotes the Expression of Proinflammatory and Th1-Type Cytokines and Delay the Onset of Mortality in Cobia (Rachycentron canadum) During Photobacterium damselae subsp. damselae Infection

The results indicate that SSWE treatment facilitates the induction of Th1-type cytokines in cobia to fight against Phdd infection and has the potential to be used as an immunostimulant and vaccine adjuvant for fish.

Genotyping and phenotyping of Lactococcus garvieae isolates from fish by pulse-field gel electrophoresis (PFGE) and electron microscopy indicate geographical and capsular variations.

The findings emphasize the importance of analysing the morphological and genetic diversity in L. garvieae being correlated for proper taxonomic classification in vaccine strain selection and epidemiological studies.

Emerging fish pathogens Lactococcus petauri and L. garvieae in Nile tilapia (Oreochromis niloticus) farmed in Brazil

Lactococcosis outbreaks in Nile tilapia on Brazilian farms were monitored from 2019 to 2022 and characterized the isolates through molecular identification of the bacterial species, multilocus sequence typing (MLST) analysis, virulence to Nile Tilapia, and antimicrobial susceptibility.



An outbreak of Lactococcus garvieae Infection in Cage-cultured Red Lip Mullet Chelon haematocheilus with Green Liver Syndrome

Examination of red lip mullet Chelon haematocheilus revealed that fatty liver syndrome with hepatocyte degeneration, reflected in heterokaryons, inflammatory lesions, and melanomacrophage centers, had caused fibrosis around the kidney, spleen, and blood vessels.

Isolation and molecular identification of the etiological agents of streptococcosis in Nile tilapia (Oreochromis niloticus) cultured in net cages in Lake Sentani, Papua, Indonesia

Experimental infections and reisolation of the bacteria from morbid and dead fish suggest they are the causative agents of streptococcosis, which rendered high mortality among cage cultured Nile tilapia in Lake Sentani.

Identification and Pathology of Lactococcus garvieae Isolated from Cultured and Wild Giant Freshwater Prawns (Macrobrachium rosenbergii de Man) in Thailand

Histopathological findings revealed severe damaged hepatopancreatic tubules caused by bacterial infection and hemocytic infiltrations observed in the infected muscle of the giant freshwater prawns experimentally infected with L. garvieae indicated depression in the prawn’s immune response.

Lactococcus garvieae infection in the giant freshwater prawn Macrobranchium rosenbergii confirmed by polymerase chain reaction and 16S rDNA sequencing.

An epizootic bacterial infection in the giant freshwater prawn Macrobranchium rosenbergii occurred in Taiwan from May to June 1999 and is the first report of confirmed L. garvieae infection in prawn aquaculture.

Comparison of genetic characteristics and pathogenicity of Lactococcus garvieae isolated from aquatic animals in Taiwan.

Seventy-six Taiwanese bacterial isolates including 74 from diseased, cultured, aquatic animals and 1 human isolate, all collected between 1999 and 2006, were confirmed by PCR assay to be Lactococcus garvieae.

Lactococcus garvieae infection of cultured rainbow trout, Oncorhynchus mykiss, in Taiwan and associated biophysical characteristics and histopathology

Five isolates of Gram positive, cocci-shaped, non-hemolytic bacteria were isolated from dis-eased rainbow trout, Oncorhynchus mykiss in Taiwan, finding lesions in the brain, heart, spleen, kidney, small intestine and eye characterized by fibroplasia at the serosa of organs.

Study of pathogenicity and severity of Lactococcus garvieae isolated from rainbow trout (Oncorhynchus mykiss) farms in Kohkilooieh and Boyerahmad province

The results of this study showed that isolated bacteria had high severity for rainbow trout, and the presence of bacteria in internal organs of suspected fish showed a severe systemic infection in challenged fish.

Genotypic diversity, and molecular and pathogenic characterization of Photobacterium damselae subsp. piscicida isolated from different fish species in Taiwan.

This study provides epidemiological data of Phdp infections in farmed fish in Taiwan, which is necessary to develop comprehensive prevention and control strategies for the disease.

Identification of Lactococcus garvieaeby PCR

The PCR assay that was developed is the only practical test besides the clindamycin test which can specifically identify the zoonotic agent L. garvieae and which can differentiate it from L. lactis.

Phenotypic and Genetic Characterization ofLactococcus garvieae Isolated in Spain from Lactococcosis Outbreaks and Comparison with Isolates of Other Countries and Sources

Examination of biotype and PFGE results indicated that the trout lactococcosis outbreaks in Spain and Portugal and those in France and Italy were produced by genetically unrelated clones.