First report of tobacco curly shoot virus infecting Malvastrum coromandelianum in China

  title={First report of tobacco curly shoot virus infecting Malvastrum coromandelianum in China},
  author={Pengbai Li and Chenchen Jing and Ke Li and Jiang Du and Gentu Wu and Mingjun Li and Xianchao Sun and Ling Qing},
  journal={Journal of Plant Pathology},
Tobacco curly shoot virus (TbCSV), a monopartite begomovirus in the family Geminiviridae, mainly infects tobacco and tomato and some weeds. To determine whether TbCSV infects Malvastrum coromandelianum, 26 samples ofM. coromandelianumwith typical foliar yellow vein symptom were collected from Sichuan and Guangxi provinces of China in 2016. Total DNA was extracted and subjected to PCR detection using degenerate primer PA/PB for geminiviruses (Deng et al. 2010). A 500 bp DNA fragment was… 


Detection and differentiation of whitefly-transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers
Summary Degenerate oligonucleotide primers, designed for amplification of an approx. 500 bp fragment of DNA-A of five well characterised whitefly-transmitted geminiviruses, were used in the
Trans-replication of, and competition between, DNA beta satellites in plants inoculated with Tomato yellow leaf curl China virus and Tobacco curly shoot virus.
It is demonstrated that both Y10 DNA beta (Y10beta) and Y35 DNAbeta (Y35beta) were stably trans-replicated by the noncognate Begomovirus, although the nonCognate DNA beta accumulated in plants at a lower level than did the cognate DNAbeta.
Universal primers for the PCR-mediated amplification of DNA β
DNA β is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper