First isolation of gamma-L-glutamyl-gamma-aminobutyric acid from bovine brains.


We had obtained some immunohistochemical indications which suggested that peptide(s) containing GABA at the C-terminal could exist in specific neurons in brains. We therefore sought such GABA conjugate(s) by isolating an acidic GABA-dipeptide from bovine brains and determined its structure as gamma-L-glutamyl-gamma-aminobutyric acid (gamma-L-Glu-gamma Abu:GluGABA). Its gamma-linkage was determined by a mass-spectrometric (B/E linked scan SIMS) analysis; its chirality was established by a chiral column HPLC technique. This peptide is the first acidic GABA-peptide recognized in brain and the first brain peptide containing GABA at the C-terminal.


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@article{Yamamoto1992FirstIO, title={First isolation of gamma-L-glutamyl-gamma-aminobutyric acid from bovine brains.}, author={Akiko Yamamoto and Kazuharu Ienaga and Kozo Nakamura and Noriko Nishimura and Kunihiko Higashiura and Yoshihiko Kurimoto and A. Inoue and H Kimurat}, journal={Neuroreport}, year={1992}, volume={3 4}, pages={330-2} }