First identification of an SHV-12 extended-spectrum beta-lactamase in Klebsiella pneumoniae isolated in Italy.

@article{Laksai2000FirstIO,
  title={First identification of an SHV-12 extended-spectrum beta-lactamase in Klebsiella pneumoniae isolated in Italy.},
  author={Y Laksai and Maria Severino and Mariagrazia Perilli and Gianfranco Amicosante and Giovanni Bonfiglio and S. T. Stefani},
  journal={The Journal of antimicrobial chemotherapy},
  year={2000},
  volume={45 3},
  pages={349-51}
}
A clinical isolate of Klebsiella pneumoniae highly resistant to third- and fourth-generation cephalosporins, cephamycins and aminoglycosides, was isolated in 1991 from urine. Analysis of a crude extract showed the presence of three beta-lactamases with isoelectric points of 6.6, 7.5 and 8.2. The enzyme with pI 8.2 was transferred by conjugation into Escherichia coli K-12 J53 and was responsible for the resistance to third-generation cephalosporins and monobactams, but not to other antibiotics… CONTINUE READING