First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens

  title={First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens},
  author={Hideo Ishii and Junko Tanoue and Michiyo Oshima and Wen-Hsin Chung and Kumiko Nishimura and Junichiro Yamaguchi and Fumihiro Nemoto and Kazuhiro So and Toshitaka Iwama and Hideaki Yoshimatsu and Motoshige Shimizu and Toru Kozawa},
  journal={Journal of General Plant Pathology},
Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high… CONTINUE READING


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