First Look at the Venom of Naja ashei

@article{Hus2018FirstLA,
  title={First Look at the Venom of Naja ashei},
  author={Konrad Kamil Hus and Justyna Buczkowicz and Vladim{\'i}r Petrilla and Monika Petrillov{\'a} and Andrzej Łyskowski and Jaroslav Leg{\'a}th and Aleksandra Bocian},
  journal={Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry},
  year={2018},
  volume={23}
}
Naja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of N. ashei with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry… Expand
Proteomic characterization of Naja mandalayensis venom
TLDR
The present study set the basis for a better comprehension of the envenomation from a molecular perspective and, by increasing the interest and information available for this species, allows future venom comparisons among cobras and their diverse venom proteins. Expand
Proteomic Investigations of Two Pakistani Naja Snake Venoms Species Unravel the Venom Complexity, Posttranslational Modifications, and Presence of Extracellular Vesicles
TLDR
The present study has shown that these snake venoms consist of a highly diversified proteome, and showed the presence of N-terminal acetylation (N-ace) of a few proteins in both venoms, which is the first study revealing this posttranslational modification in snake venom. Expand
Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species
TLDR
Antifungal activity of snake venoms should be studied with great caution and a plethora of pathogenic biomarkers should be considered in the future experiments. Expand
Distinctive Distribution of Secretory Phospholipases A2 in the Venoms of Afro-Asian Cobras (Subgenus: Naja, Afronaja, Boulengerina and Uraeus)
TLDR
The negligible PLA2 activity in Uraeus cobra venoms implies that PLA2 may not be ubiquitous in all snake venoms, and may potentially influence the efficacy of cobra antivenom in specific use for venom neutralization. Expand
Enzymatic activity and brine shrimp lethality of venom from the large brown spitting cobra (Naja ashei) and its neutralization by antivenom
TLDR
Intermediate and high doses of the venom exhibited similar mortalities in brine shrimp and test antivenoms were generally non-toxic but poorly neutralized svPLA2 activity, while Mexican manufactured antivenom had lower protein content but neutralized venom-induced brinerimp lethality much more effectively than Indian manufacturedAntivenom. Expand
Different Research Approaches in Unraveling the Venom Proteome of Naja ashei
TLDR
A rapid and straightforward technique for the separation of the fraction of proteins from the three-finger toxin family is reported, which underline the necessary caution in the interpretation of data based on a comparative analysis of data derived from different studies. Expand
Antimicrobial Activity of Protein Fraction from Naja ashei Venom against Staphylococcus epidermidis
TLDR
A new group of low abundant proteins containing the Ig-like domain that have not been previously described in snake venoms are identified, showing exceptional antimicrobial effects both alone and in combination with antibiotics. Expand
Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity
TLDR
The results show that the mechanism of protein–lipid interactions depends on both the presence of lipid polar parts (especially zwitterionic type of lipids) and the degree of membrane saturation (the greatest-for unsaturated lipids). Expand
Comparative venomics and preclinical efficacy evaluation of a monospecific Hemachatus antivenom towards sub-Saharan Africa cobra venoms.
TLDR
Comparative proteomics and third generation antivenomics provide clues for the design of optimum venom mixtures for the preparation of broad spectrum antivenoms and suggest a closer kinship of H. haemachatus and Naja subgenus Afrocobra spitting cobras than to NajaSubgenus Uraeus neurotoxic taxa. Expand
The Relative Efficacy of Chemically Diverse Small-Molecule Enzyme-Inhibitors Against Anticoagulant Activities of African Spitting Cobra (Naja Species) Venoms
TLDR
Small molecule inhibitors (SMIs) might hold great promise as initial, field-based, treatments for snakebite envenoming as well as addressing fundamental limitations of antivenom in the clinical setting where certain toxin effects are unneutralized. Expand
...
1
2
...

References

SHOWING 1-10 OF 60 REFERENCES
Proteomic characterization of two snake venoms: Naja naja atra and Agkistrodon halys.
TLDR
Functional analysis based upon toxin categories reveals that cobra venom has a high abundance of cardio- and neurotoxins, whereas viper venom contains a significant amount of haemotoxins and metalloproteinases, suggesting that post-translational modification may be a significant characteristic of venomous proteins. Expand
Venom-gland transcriptome and venom proteome of the Malaysian king cobra (Ophiophagus hannah)
TLDR
The diversity and abundance of toxins in the venom of the Malaysian king cobra (MOh) are reported, and the results correlate with the pathophysiological actions of MOh venom, and dispute the use of Naja cobra antivenoms to treat MOh envenomation. Expand
Snake venomics of African spitting cobras: toxin composition and assessment of congeneric cross-reactivity of the pan-African EchiTAb-Plus-ICP antivenom by antivenomics and neutralization approaches.
TLDR
The ability of EchiTAb-Plus-ICP antivenom to immunodeplete and neutralize the venoms of African spitting cobras was assessed by antivenomics and neutralization tests and the lack of neutralization of lethality of N. nubiae venom may be of medical relevance only in relatively populous areas of the Saharan region. Expand
Proteomic Analyses of Agkistrodon contortrix contortrix Venom Using 2D Electrophoresis and MS Techniques
TLDR
Ten groups of proteins present in the venom are identified, of which phospholipase A2 and metalloprotease and serine proteases constitute the largest groups, and for the first time presence of 5′-nucleotidase in venom was found in this group of snakes. Expand
Proteome and Peptidome of Vipera berus berus Venom
TLDR
An attempt was made to identify proteins and peptides derived from this species venom by using high resolution two-dimensional electrophoresis and MALDI ToF/ToF mass spectrometry, and all successfully identified peptides belong to the bradykinin-potentiating peptides family. Expand
Structural considerations of the snake venom metalloproteinases, key members of the M12 reprolysin family of metalloproteinases.
  • J. Fox, S. Serrano
  • Biology, Medicine
  • Toxicon : official journal of the International Society on Toxinology
  • 2005
TLDR
What the reader will observe is that there are very interesting structural features displayed by the various SVMP classes and subclasses that provide insight into their functional characteristics. Expand
Purification of a phospholipase A2 from Daboia russelii siamensis venom with anticancer effects
TLDR
The Drs-PLA2 had indirect hemolytic and anticoagulant activities, cytotoxic activity with a CC50 of 65.8nM, and inhibited SK-MEL-28 cell migration with an IC50 of 25.6nM. Expand
Carbon monoxide inhibits hemotoxic activity of Elapidae venoms: potential role of heme
TLDR
Data demonstrate indirectly that the biometal heme is likely bound to these disparate venoms as an intermediary modulatory molecule and may be a potential modulator of heme as a protective mechanism for venomous snakes against injury from their own proteolytic venoms. Expand
5'-Nucleotidase from Vipera lebetina venom.
TLDR
The cloning and sequencing of the 5'-NT coding cDNA resulted in 5'-truncated construct, and MALDI-TOF and Orbitrap mass-spectrometry of the tryptic peptides confirmed the translated N-terminally truncated protein sequence concordance to the5'-NT isolated from the venom. Expand
Observations on the bite of the Mozambique spitting cobra (Naja mossambica mossambica).
  • C. Tilbury
  • Medicine
  • South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde
  • 1982
TLDR
The efficacy of the SAIMR's polyvalent antivenom was tested, and the evidence suggests that its effectiveness is indirectly proportional to the time which has elapsed between the bite and the administration of the antivenoms. Expand
...
1
2
3
4
5
...