First Glycoside Hydrolase Family 2 Enzymes from Thermus antranikianii and Thermus brockianus with β-Glucosidase Activity

  title={First Glycoside Hydrolase Family 2 Enzymes from Thermus antranikianii and Thermus brockianus with $\beta$-Glucosidase Activity},
  author={Carola Schr{\"o}der and Saskia Blank and Garabed Antranikian},
  journal={Frontiers in Bioengineering and Biotechnology},
Two glycoside hydrolase encoding genes (tagh2 and tbgh2) were identified from different Thermus species using functional screening. Based on amino acid similarities, the enzymes were predicted to belong to glycoside hydrolase (GH) family 2. Surprisingly, both enzymes (TaGH2 and TbGH2) showed twofold higher activities for the hydrolysis of nitrophenol-linked β-D-glucopyranoside than of -galactopyranoside. Specific activities of 3,966 U/mg for TaGH2 and 660 U/mg for TbGH2 were observed. In… 

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