Firefly luciferase produces hydrogen peroxide as a coproduct in dehydroluciferyl adenylate formation.

@article{Fraga2006FireflyLP,
  title={Firefly luciferase produces hydrogen peroxide as a coproduct in dehydroluciferyl adenylate formation.},
  author={Hugo Fraga and Diogo Fernandes and Jiri Novotny and Rui Fontes and Joaquim C G Esteves da Silva},
  journal={Chembiochem : a European journal of chemical biology},
  year={2006},
  volume={7 6},
  pages={929-35}
}
Firefly luciferase catalyzes the synthesis of H2O2 from the same substrates as the bioluminescence reaction: ATP and luciferin (D-LH2). About 80% of the enzyme-bound intermediate D-luciferyl adenylate (D-LH2-AMP) is oxidized into oxyluciferin, and a photon is emitted during this reaction. The enzyme pathway responsible for the generation of H2O2 is a side reaction in which D-LH2-AMP is oxidized into dehydroluciferyl adenylate (L-AMP). Like the bioluminescence reaction, the luciferase-catalyzed… CONTINUE READING
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