Firefly luciferase is a bifunctional enzyme: ATP-dependent monooxygenase and a long chain fatty acyl-CoA synthetase.

@article{Oba2003FireflyLI,
  title={Firefly luciferase is a bifunctional enzyme: ATP-dependent monooxygenase and a long chain fatty acyl-CoA synthetase.},
  author={Yuichi Oba and Makoto Ojika and Satoshi Inouye},
  journal={FEBS letters},
  year={2003},
  volume={540 1-3},
  pages={251-4}
}
Firefly luciferase can catalyze the formation of fatty acyl-CoA via fatty acyl-adenylate from fatty acid in the presence of ATP, Mg(2+) and coenzyme A (CoA). A long chain fatty acyl-CoA (C(16)-C(20)), produced by luciferase from a North American firefly (Photinus pyralis) and a Japanese firefly (Luciola cruciata), was isolated and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis. Of a number of substrates tested, linolenic acid (C(18:3)) and… CONTINUE READING