Firefly luciferase gene: structure and expression in mammalian cells.

@article{Wet1987FireflyLG,
  title={Firefly luciferase gene: structure and expression in mammalian cells.},
  author={Juanita de Wet and Keith V. Wood and Marcella Deluca and D. R. Helinski and Suresh Subramani},
  journal={Molecular and cellular biology},
  year={1987},
  volume={7 2},
  pages={725-37}
}
The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined from the analysis of cDNA and genomic clones. The gene contains six introns, all less than 60 bases in length. The 5' end of the luciferase mRNA was determined by both S1 nuclease analysis and primer extension. Although the luciferase cDNA clone lacked the six N… CONTINUE READING