Fine tuning of an anti-testosterone antibody binding site by stepwise optimisation of the CDRs.

@article{Hemminki1998FineTO,
  title={Fine tuning of an anti-testosterone antibody binding site by stepwise optimisation of the CDRs.},
  author={Ari Hemminki and Seija Niemi and L Hautoniemi and Hans S{\"o}derlund and Kristiina Takkinen},
  journal={Immunotechnology : an international journal of immunological engineering},
  year={1998},
  volume={4 1},
  pages={59-69}
}
BACKGROUND We have previously reported specificity improvement of an anti-testosterone monoclonal antibody (3-C4F5) by random mutagenesis of the third complementarity determining regions (CDR3s) and by phage display selection. OBJECTIVES Here we extend the mutagenesis strategy to the other CDRs and select the mutant libraries using two different approaches in order to further fine-tune the binding properties of this recombinant Fab fragment. STUDY DESIGN To improve the affinity the new… CONTINUE READING