Fine structure of heparan sulfate regulates syndecan-1 function and cell behavior.

@article{Sanderson1994FineSO,
  title={Fine structure of heparan sulfate regulates syndecan-1 function and cell behavior.},
  author={Ralph D. Sanderson and Jeremy. E. Turnbull and John Thomas Gallagher and Arthur D. Lander},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 18},
  pages={13100-6}
}
Two myeloma cell lines, MPC-11 and P3X63Ag8.653 (P3), have almost identical amounts of syndecan-1 at their cell surface. The syndecan-1 molecules from both lines are similar in size, have indistinguishable core proteins, and have similarly sized heparan sulfate chains. Nevertheless, syndecan-1 on MPC-11 mediates cell adhesion to type I collagen, whereas P3 cells do not bind collagen. Affinity co-electrophoresis reveals that intact syndecan-1 isolated from P3 cells binds collagen poorly and that… CONTINUE READING