Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice

@article{Hittalmani2000FineMA,
  title={Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice},
  author={Shailaja Hittalmani and Arnold S. Parco and Teresita Mew and Robert S. Zeigler and Ning Huang},
  journal={Theoretical and Applied Genetics},
  year={2000},
  volume={100},
  pages={1121-1128}
}
Three major genes (Pi1, Piz-5 and Pita) for blast resistance on chromosomes 11, 6 and 12, respectively, were fine-mapped and closely linked RFLP markers identified. New markers for Pi1 and Pita were found that were flanking the genes. The three genes were pyramided using RFLP markers. A PCR-based SAP (sequence amplified polymorphism) marker was used to identify Piz-5 in the segregating population. The plants carrying the two- and three-gene combinations that were tested for resistance to leaf… CONTINUE READING
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