Filter centrifugation as a sampling method for miniaturization of extracellular fungal enzyme activity measurements in solid media

@inproceedings{Heinonsalo2012FilterCA,
  title={Filter centrifugation as a sampling method for miniaturization of extracellular fungal enzyme activity measurements in solid media},
  author={Jussi Heinonsalo and Grit Kabiersch and Riitta Maarit Niemi and Suvi Simpanen and Mets{\"a}ntutkimuslaitos and Martin Hofrichter and Annele Hatakka and Kari Timo Steffen},
  year={2012}
}
A novel sampling method to evaluate extracellular fungal enzyme activities was developed and the validity tested for agar media. The method is based on centrifugation of small agar pieces taken from growing fungal solid-state cultures. Centrifuge tubes that allow spinning liquid out from small samples containing, for example, the hyphal front of a growing mycelium are essential for the protocol. Centrifugation recovers a liquid phase from the samples, which contains soluble material including… CONTINUE READING

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