Filopodia rotate and coil by actively generating twist in their actin shaft

@article{Leijnse2022FilopodiaRA,
  title={Filopodia rotate and coil by actively generating twist in their actin shaft},
  author={Natascha Leijnse and Younes F Barooji and Mohammad Reza Arastoo and Stine Lauritzen S{\o}nder and Bram Verhagen and Lena Wullkopf and Janine Terra Erler and Szabolcs Semsey and Jesper Nylandsted and Lene Broeng Oddershede and Amin Doostmohammadi and Poul Martin Bendix},
  journal={Nature Communications},
  year={2022},
  volume={13}
}
Filopodia are actin-rich structures, present on the surface of eukaryotic cells. These structures play a pivotal role by allowing cells to explore their environment, generate mechanical forces or perform chemical signaling. Their complex dynamics includes buckling, pulling, length and shape changes. We show that filopodia additionally explore their 3D extracellular space by combining growth and shrinking with axial twisting and buckling. Importantly, the actin core inside filopodia performs a… 

References

SHOWING 1-10 OF 75 REFERENCES
Helical buckling of actin inside filopodia generates traction
TLDR
A previously unidentified mechanism by which a cell can use rotation of the filopodial actin shaft to induce coiling and hence axial shortening of the Filopodian actin bundle is suggested.
Dynamic buckling of actin within filopodia
TLDR
There is a clear correlation between presence of actin near the tip and exertion of a traction force, thus demonstrating that the traction force is transduced along the actin shaft inside the filopodium.
An updated look at actin dynamics in filopodia
TLDR
Although force measurements have indicated a step‐like behavior in filopodial pulling, no direct evidence has been provided to link this behavior to a molecular motor like myosin, therefore, the underlying biochemical and mechanical mechanisms behind filopadial force generation still remain to be resolved.
Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella
TLDR
Findings in fish fibroblasts support the idea that filaments generated in filopodia and lamellipodia for protrusion are recycled for seeding actomyosin arrays for use in retraction.
Filopodial retraction force is generated by cortical actin dynamics and controlled by reversible tethering at the tip
TLDR
The dynamics of filopodial extension and retraction are determined by the difference between the actin polymerization rate at the tip and the retrograde flow at the base of the filopodium, reminiscent of a process used by pathogens to invade cells.
How filopodia pull: What we know about the mechanics and dynamics of filopodia
TLDR
This review summarizes current advancements in the understanding of the mechanics and dynamics of filopodia, with a focus on the molecular mechanisms behind Filopodial force exertion.
Regulated Actin Cytoskeleton Assembly at Filopodium Tips Controls Their Extension and Retraction
TLDR
Observing the movement of fiduciary marks on actin filaments in growth cones of a neuroblastoma cell line found that filopodium extension and retraction are governed by a balance between the rate of actin cytoskeleton assembly at the tip and retrograde flow.
mDia1 senses both force and torque during F-actin filament polymerization
TLDR
It is found that pulling force on an actin filament promoted faster actin polymerisation by the formin mDia1, and also found that the act in filament must be torsionally unconstrained, suggesting that mDIA1 can also sense torque.
...
1
2
3
4
5
...