Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic.

@article{Gomes2017FieldVO,
  title={Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic.},
  author={Ciro Martins Gomes and Mariana Vicente Cesetti and Nat{\'a}lia Aparecida de Paula and Sebastian Vernal and Gaurav Gupta and Raimunda Nonata Ribeiro Sampaio and Ana Maria Roselino},
  journal={Journal of clinical microbiology},
  year={2017},
  volume={55 2},
  pages={526-534}
}
The precise diagnosis of American tegumentary leishmaniasis (ATL) is an essential task due to the disease's associated morbidity. A noninvasive, extremely sensitive, and highly specific exam is critical, particularly for mucosal leishmaniasis (ML), in which a low parasite quantity is expected. We aimed to compare the diagnostic accuracy of swab and biopsy sample analysis using SYBR Green- and TaqMan-based real-time PCR (qPCR) assays with that of a composite reference standard consisting of the… CONTINUE READING
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