Field Assessment of Outcrossing from Transgenic Pea (Pisum Sativum L.) Plants

@article{Polowick2002FieldAO,
  title={Field Assessment of Outcrossing from Transgenic Pea (Pisum Sativum L.) Plants},
  author={Patricia L. Polowick and Albert Vandenberg and John D. Mahon},
  journal={Transgenic Research},
  year={2002},
  volume={11},
  pages={515-519}
}
The frequency of outcrossing from a transgenic line of peas into three cultivars ('Carneval', 'Montana', 'Tipu') was studied in the field in 1997 and 1999. Two dominant traits, normal leaf form and a highly-expressed β-glucuronidase (gusA) gene, were used as markers of pollen transfer. Because of heterogeneity in the commercial seed sources, leaf form alone was unreliable for assessing pollen migration into 'trap' plants. Of approximately 9000 offspring tested, only five plants scored positive… CONTINUE READING

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