Fidelity of polymerase chain reaction-direct sequencing analysis of damaged forensic samples.

Abstract

Polymerase chain reaction (PCR) direct sequence analysis was performed on aged forensic samples, six or thirteen years old. This method allowed unambiguous genetic typing, but PCR products from such samples showed several artifacts. Control samples generated sequence ambiguities at a frequency of 1 in 567 bases, but the aged samples had an error frequency… (More)

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@article{Fattorini1999FidelityOP, title={Fidelity of polymerase chain reaction-direct sequencing analysis of damaged forensic samples.}, author={Paolo Fattorini and R Ciofuli and F Cossutta and Piero Giulio Giulianini and Paolo Edomi and Marco Furlanut and Carlo Previder{\'e}}, journal={Electrophoresis}, year={1999}, volume={20 17}, pages={3349-57} }