Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.

  title={Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.},
  author={Kenneth R Tindall and T. A. Kunkel},
  volume={27 16},
We have determined the fidelity of in vitro DNA synthesis catalyzed at high temperature by the DNA polymerase from the thermophilic bacterium Thermus aquaticus. Using a DNA substrate that contains a 3'-OH terminal mismatch, we demonstrate that the purified polymerase lacks detectable exonucleolytic proofreading activity. The fidelity of the Taq polymerase was measured by two assays which score errors produced during in vitro DNA synthesis of the lacZ alpha complementation gene in M13mp2 DNA. In… CONTINUE READING
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