Fidelity of DNA sequence recognition by the SfiI restriction endonuclease is determined by communications between its two DNA-binding sites.

@article{Bellamy2008FidelityOD,
  title={Fidelity of DNA sequence recognition by the SfiI restriction endonuclease is determined by communications between its two DNA-binding sites.},
  author={Stuart R. W. Bellamy and Petros Mina and Susan E. Retter and Stephen E. Halford},
  journal={Journal of molecular biology},
  year={2008},
  volume={384 3},
  pages={
          557-63
        }
}
The SfiI restriction endonuclease is a tetramer in which two subunits form a dimeric unit that contains one DNA binding cleft and the other two subunits contain a second cleft on the opposite side of the protein. Full activity requires both clefts to be filled with its recognition sequence: SfiI has low activity when bound to one site. The ability of SfiI to cleave non-cognate sites, one base pair different from the true site, was initially tested on substrates that lacked specific sites but… CONTINUE READING
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