Corpus ID: 26794976

Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae.

  title={Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae.},
  author={K. Shimizu and K. Hashimoto and J. Kirchner and W. Nakai and H. Nishikawa and M. Resnick and A. Sugino},
  journal={The Journal of biological chemistry},
  volume={277 40},
  • K. Shimizu, K. Hashimoto, +4 authors A. Sugino
  • Published 2002
  • Medicine
  • The Journal of biological chemistry
  • DNA polymerases delta and epsilon (pol delta and epsilon) are the major replicative polymerases and possess 3'-5' proofreading exonuclease activities that correct errors arising during DNA replication in the yeast Saccharomyces cerevisiae. This study measures the fidelity of the holoenzyme of wild-type pol epsilon, the 3'-5' exonuclease-deficient pol2-4, a +1 frameshift mutator for homonucleotide runs, pol2C1089Y, and pol2C1089Y pol2-4 enzymes using a synthetic 30-mer primer/100-mer template… CONTINUE READING
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