Fibronectin and asialoglyprotein receptor mediate hepatitis B surface antigen binding to the cell surface

Abstract

Both fibronectin and the asialoglycoprotein receptor (ASGPR) have been identified by some investigators as partners for hepatitis B virus (HBV) envelope proteins. Because fibronectin is a natural ligand for ASGPR, we speculated that HBV might attach to ASGPR expressed on the hepatocyte surface via fibronectin. To test this hypothesis, we first confirmed by co-immunoprecipitation that ASGPR, fibronectin and HBsAg bind to each other in HepG2.2.15 cells, and possible binding domains were identified by GST pull-down. In addition, by measuring binding of HBsAg to cells, we found that ASGPR and fibronectin enhanced the binding capability of HBsAg to HepG2 cells, and even to 293T and CHO cells, which normally do not bind HBV. In conclusion, our findings suggest that both fibronectin and ASGPR mediate HBsAg binding to the cell surface, which provides further evidence for the potential roles of these two proteins in mediating HBV binding to liver cells.

DOI: 10.1007/s00705-010-0657-5

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Cite this paper

@article{Yang2010FibronectinAA, title={Fibronectin and asialoglyprotein receptor mediate hepatitis B surface antigen binding to the cell surface}, author={Jing Yang and Feng Wang and Linlin Tian and Jing Su and Xiangqian Zhu and Li Lin and Xiaoran Ding and Xuejun Wang and Shengqi Wang}, journal={Archives of Virology}, year={2010}, volume={155}, pages={881-888} }