Fibroblast growth factor induces primitive streak formation in rabbit pre-implantation embryos in vitro

@article{Angelis1993FibroblastGF,
  title={Fibroblast growth factor induces primitive streak formation in rabbit pre-implantation embryos in vitro},
  author={Martin Hrab{\'e} de Angelis and Ch. Kirchner},
  journal={Anatomy and Embryology},
  year={1993},
  volume={187},
  pages={269-273}
}
Culturing of rabbit pre-implantation embryos was performed in Ham's F10 medium supplemented with polyvinylpyrrolidone. Under these culture conditions, day 6 post coitum blastocysts increased their diameter within 24 h to 80% of that of day 7 blastocysts grown in vivo. Despite this substained growth, the embryonic disc remained undifferentiated with clear signs of degeneration after 24 h of culture. Basic fibroblast growth factor (bFGF) was able to overcome this developmental block. After 12 h… CONTINUE READING