Fiber type-specific distribution of parvalbumin in rabbit skeletal muscle

@article{Schmitt1991FiberTD,
  title={Fiber type-specific distribution of parvalbumin in rabbit skeletal muscle},
  author={Tom Schmitt and Dirk Pette},
  journal={Histochemistry},
  year={1991},
  volume={96},
  pages={459-465}
}
A highly sensitive sandwich ELISA for parvalbumin (PA), based on a fluorometric detection system, was developed. This assay detected PA concentrations as low as 20 pg/ml (2 pg per assay) and was used for measuring PA contents in fragments of single muscle fibers isolated from freeze-dried 100–150 μm thick cross sections. The fibers were typed according to their histo-chemically assessed mATPase in parallel cross sections. Type I fibers from rabbit tibialis anterior (TA) and vastus lateralis (VL… CONTINUE READING

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