Fhit proteins can also recognize substrates other than dinucleoside polyphosphates

@article{Guranowski2008FhitPC,
  title={Fhit proteins can also recognize substrates other than dinucleoside polyphosphates},
  author={A. Guranowski and A. Wojdyła and M. Pietrowska-Borek and P. Bieganowski and E. N. Khurs and M. Cliff and G. Blackburn and Damian Błaziak and W. Stec},
  journal={FEBS Letters},
  year={2008},
  volume={582}
}
We show here that Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5′‐monophosphates from such natural metabolites as adenosine 5′‐phosphosulfate and adenosine 5′‐phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5′‐O‐phosphorofluoridate and adenosine 5′‐O‐(γ‐fluorotriphosphate), and release AMP from them. With respect to the former… Expand
Recognition of different nucleotidyl-derivatives as substrates of reactions catalyzed by various HIT-proteins
Proteins that have a histidine triad in their active sites belong to the HIT-protein superfamily. They are ubiquitous, are involved in the metabolism of different nucleotides and catalyze theirExpand
Novel reactivity of Fhit proteins: catalysts for fluorolysis of nucleoside 5'-phosphoramidates and nucleoside 5'-phosphosulfates to generate nucleoside 5'-phosphorofluoridates.
TLDR
Substrate specificity and kinetic characterization of the fluorolytic reactions catalysed by the human Fhit and other examples of involvement of fluoride in the biochemistry of nucleotides are described. Expand
Dual activity of certain HIT‐proteins: A. thaliana Hint4 and C. elegans DcpS act on adenosine 5′‐phosphosulfate as hydrolases (forming AMP) and as phosphorylases (forming ADP)
TLDR
These proteins, and in particular Hint4, also catalyzed hydrolysis or phosphorolysis of some other adenylyl‐derivatives but at lower rates than those for APS cleavage, and the possible role of some HIT‐proteins in APS metabolism is discussed. Expand
Hint2, the mitochondrial nucleoside 5'-phosphoramidate hydrolase; properties of the homogeneous protein from sheep (Ovis aries) liver.
TLDR
Studies of the rates of hydrolysis of different NH2-pA derivatives show that Hint2 is more specific towards compounds with a P-N bond than Hint1, and the thermostability of these two proteins is compared. Expand
The reaction of activated RNA species with aqueous fluoride ion: a convenient synthesis of nucleotide 5′-phosphorofluoridates and a note on the mechanism
The chemistry of 5′-phosphorimidazolides of ribonucleosides is extended to include their reaction with alkali metal fluorides in aqueous solution. High yields of 5′-phosphorofluoridates are formed,Expand
Binding and catalysis of Humulus lupulus adenylate isopentenyltransferase for the synthesis of isopentenylated diadenosine polyphosphates
TLDR
Judging by the existing biosynthetic and hydrolytic systems for Ap n A in plants, Apn A and isopentenyl‐Ap n A may occur in the plant cells, with functional importance. Expand
A fluorescent HTS assay for phosphohydrolases based on nucleoside 5'-fluorophosphates: its application in screening for inhibitors of mRNA decapping scavenger and PDE-I.
TLDR
The assay presented here is the first fluorescence-based HTS-adaptable assay for DCPS, an established therapeutic target for spinal muscular atrophy, and should be useful for phosphohydrolase specificity profiling and inhibitor discovery, particularly in the context of DcpS and other HIT-family enzymes. Expand
Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins
TLDR
It is described how the search for the transferase led to this serendipitous finding and basic kinetic parameters of ammonolytic reactions catalysed by human Fhit are provided. Expand
Plant nucleoside 5'-phosphoramidate hydrolase; simple purification from yellow lupin (Lupinus luteus) seeds and properties of homogeneous enzyme.
TLDR
Mass spectrometric analysis showed that the lupin hydrolase exhibits closest similarity to Arabidopsis thaliana Hint1 protein, and its ability to split the P-S bond in adenosine 5'-phosphorothioate, is typical of known Hints1 proteins. Expand
Exogenous adenosine 5'-phosphoramidate behaves as a signal molecule in plants; it augments metabolism of phenylpropanoids and salicylic acid in Arabidopsis thaliana seedlings.
TLDR
It is shown that adenosine 5'-phosphoramidate, whose level can be controlled by HIT-family proteins, induced similar effects and was postulated to be considered as a novel signaling molecule, similar to diadenosine polyphosphates and cyclic nucleotides. Expand
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