Fewer essential genes in mycoplasmas than previous studies suggest.

@article{Dybvig2010FewerEG,
  title={Fewer essential genes in mycoplasmas than previous studies suggest.},
  author={Kevin F Dybvig and P .D .R Lao and David Starr Jordan and Warren L. Simmons},
  journal={FEMS microbiology letters},
  year={2010},
  volume={311 1},
  pages={
          51-5
        }
}
Here, we describe mutants of Mycoplasma pulmonis that were obtained using a minitransposon, Tn4001TF1, which actively transposes but is then unable to undergo subsequent excision events. Using Tn4001TF1, we disrupted 39 genes previously thought to be essential for growth. Thus, the number of genes required for growth has been overestimated. This study also revealed evidence of gene duplications in M. pulmonis and identified chromosome segregation proteins that are dispensable in mycoplasmas but… CONTINUE READING
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