Fewer essential genes in mycoplasmas than previous studies suggest.


Here, we describe mutants of Mycoplasma pulmonis that were obtained using a minitransposon, Tn4001TF1, which actively transposes but is then unable to undergo subsequent excision events. Using Tn4001TF1, we disrupted 39 genes previously thought to be essential for growth. Thus, the number of genes required for growth has been overestimated. This study also… (More)
DOI: 10.1111/j.1574-6968.2010.02078.x


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