Fetal rat sympathetic neurons maintained in a serum-free medium retain induced cholinergic characteristics.

@article{Higgins1984FetalRS,
  title={Fetal rat sympathetic neurons maintained in a serum-free medium retain induced cholinergic characteristics.},
  author={Dennis Higgins and Lorraine Iacovitti and Harold Burton},
  journal={Brain research},
  year={1984},
  volume={316 1},
  pages={71-82}
}
When maintained in some serum-containing media, fetal rat sympathetic neurons acquire substantial choline acetyltransferase activity and form cholinergic synapses in vitro. However, when they are maintained in a serum-free, defined culture medium, choline acetyltransferase activity is not detected and cholinergic synapses are not observed. In this study, we have examined the effects of various times of exposure to a medium inducing cholinergic function on the properties of neurons subsequently… CONTINUE READING