Fetal and adult human skin fibroblasts display intrinsic differences in contractile capacity

  title={Fetal and adult human skin fibroblasts display intrinsic differences in contractile capacity},
  author={V{\'e}ronique J. Moulin and Betty Y. Y. Tam and G Castilloux and François A Auger and Maureen D. O’Connor-McCourt and Anie Philip and Lucie Germain},
  journal={Journal of Cellular Physiology},
One of the differences between fetal and adult skin healing is the unique ability of fetal wounds to heal without contracture and scar formation. Studies have shown that the ratio between the three isoforms of TGFβ is different in adult and fetal wounds. Thus, we analyzed the capacity of adult and fetal human skin fibroblasts to contract collagen gels after stimulation with TGFβ isoforms. In control medium, fetal fibroblasts had a contractile capacity similar to that of adult fibroblasts… 

The Contractile Phenotype of Dermal Fetal Fibroblasts in Scarless Wound Healing

Understanding the biomechanical mechanisms that regulate the contractile phenotype of fetal fibroblasts may unlock new avenues for anti-scarring therapies that target myofibroblast differentiation of adult fibro Blasts.

Differential proliferative response of fetal and adult human skin fibroblasts to transforming growth factor‐β

There is a major radical in the proliferative response of fetal and adult human skin fibroblasts to TGF‐β, possibly reflecting the different repair strategies followed in these two stages of development.

Fetal dermal fibroblasts retain a hyperactive migratory and contractile phenotype under 2-and 3-dimensional constraints compared to normal adult fibroblasts.

The hyperactive migratory and contractile dynamics of fetal fibroblasts are qualitatively and quantitatively conserved despite transitions from individual cells to whole populations and from 2D to 3D constraints.

Higher TRIP-1 level explains diminished collagen contraction ability of fetal versus adult fibroblasts.

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In vitro cultured fetal fibroblasts have myofibroblast-associated characteristics and produce a fibrotic-like environment upon stimulation with TGF-β1: Is there a thin line between fetal scarless healing and fibrosis?

In vitro cultured fetal fibroblasts have myofibroblast-associated characteristics and do produce a fibrotic environment, and findings correlate with the in vivo situation.

Differential regulation of free‐floating collagen gel contraction by human fetal and adult dermal fibroblasts in response to prostaglandin E2 mediated by an EP2/cAMP‐dependent mechanism

The results indicate that fetal fibroblast contraction is maintained by a more stable actin cytoskeleton, suggesting that the migratory phenotype may be sufficient for physical remodeling of the wound matrix leading to regenerative repair.

Tissue-engineered fetal dermal matrices

Comparison of these dermal constructs to those prepared with neonatal dermal cells, adult skin, neonatal foreskin, and mid-gestation fetal skin demonstrates that these fetal dermal matrices bear marked morphological and biochemical resemblance to the mid- gestation fetal dermis.

Cellular and molecular mechanisms involved in scarless wound healing in the fetal skin

This work has shown that in vitro cultured fetal fibroblasts produce a fibrotic-like environment and the effects of stretch on myofibroblast differentiation and reduced homing is causing the deficiency of immune cells in second-trimester fetal skin.

Prostaglandin E2 differentially modulates human fetal and adult dermal fibroblast migration and contraction: implication for wound healing

Fetal dermal fibroblasts are partially refractory to the effects of PGE2, a major inflammatory mediator associated with dermal wound healing, which may have significant and specific relevance to the scarless fetal wound‐healing phenotype.



Fetal and postnatal sera differentially modulate human dermal fibroblast phenotypic and functional features in vitro

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In vitro analysis of fetal fibroblast collagenolytic activity

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Differential response of fetal and adult fibroblasts to cytokines: cell migration and hyaluronan synthesis.

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Human wound healing fibroblasts have greater contractile properties than dermal fibroblasts.

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Phenotypic and functional features of myofibroblasts in sheep fetal wounds.

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Exogenous Transforming Growth Factor‐Beta Amplifies Its Own Expression and Induces Scar Formation in a Model of Human Fetal Skin Repair

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Modulated response to cytokines of human wound healing myofibroblasts compared to dermal fibroblasts.

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The Inhibition of Fibroblast‐Populated Collagen Lattice Contraction by Human Amniotic Fluid: A Chronologic Examination

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Differences between adult and foetal fibroblasts in the regulation of hyaluronate synthesis: correlation with migratory activity.

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