Fetal and adult human skin fibroblasts display intrinsic differences in contractile capacity

@article{Moulin2001FetalAA,
  title={Fetal and adult human skin fibroblasts display intrinsic differences in contractile capacity},
  author={V{\'e}ronique J. Moulin and Betty Y. Y. Tam and G Castilloux and François A Auger and Maureen D. O’Connor-McCourt and Anie Philip and Lucie Germain},
  journal={Journal of Cellular Physiology},
  year={2001},
  volume={188}
}
One of the differences between fetal and adult skin healing is the unique ability of fetal wounds to heal without contracture and scar formation. Studies have shown that the ratio between the three isoforms of TGFβ is different in adult and fetal wounds. Thus, we analyzed the capacity of adult and fetal human skin fibroblasts to contract collagen gels after stimulation with TGFβ isoforms. In control medium, fetal fibroblasts had a contractile capacity similar to that of adult fibroblasts… 
The differential proliferative response of fetal and adult human skin fibroblasts to TGF-β is retained when cultured in the presence of fibronectin or collagen.
TLDR
The interplay between TGF-β and ECM supports the pleiotropic nature of this growth factor, in concordance with the different repair strategies between fetuses and adults.
The Contractile Phenotype of Dermal Fetal Fibroblasts in Scarless Wound Healing
TLDR
Understanding the biomechanical mechanisms that regulate the contractile phenotype of fetal fibroblasts may unlock new avenues for anti-scarring therapies that target myofibroblast differentiation of adult fibro Blasts.
Differential proliferative response of fetal and adult human skin fibroblasts to transforming growth factor‐β
TLDR
There is a major radical in the proliferative response of fetal and adult human skin fibroblasts to TGF‐β, possibly reflecting the different repair strategies followed in these two stages of development.
Fetal dermal fibroblasts retain a hyperactive migratory and contractile phenotype under 2-and 3-dimensional constraints compared to normal adult fibroblasts.
TLDR
The hyperactive migratory and contractile dynamics of fetal fibroblasts are qualitatively and quantitatively conserved despite transitions from individual cells to whole populations and from 2D to 3D constraints.
Higher TRIP-1 level explains diminished collagen contraction ability of fetal versus adult fibroblasts.
TLDR
It is concluded that human lung fetal fibroblasts are less able to contract collagen than adult lung fibro Blasts, due to their higher level of TRIP-1 expression, a novel modulator of fibroblast collagen contraction.
In vitro cultured fetal fibroblasts have myofibroblast-associated characteristics and produce a fibrotic-like environment upon stimulation with TGF-β1: Is there a thin line between fetal scarless healing and fibrosis?
TLDR
In vitro cultured fetal fibroblasts have myofibroblast-associated characteristics and do produce a fibrotic environment, and findings correlate with the in vivo situation.
Differential regulation of free‐floating collagen gel contraction by human fetal and adult dermal fibroblasts in response to prostaglandin E2 mediated by an EP2/cAMP‐dependent mechanism
TLDR
The results indicate that fetal fibroblast contraction is maintained by a more stable actin cytoskeleton, suggesting that the migratory phenotype may be sufficient for physical remodeling of the wound matrix leading to regenerative repair.
Tissue-engineered fetal dermal matrices
TLDR
Comparison of these dermal constructs to those prepared with neonatal dermal cells, adult skin, neonatal foreskin, and mid-gestation fetal skin demonstrates that these fetal dermal matrices bear marked morphological and biochemical resemblance to the mid- gestation fetal dermis.
Cellular and molecular mechanisms involved in scarless wound healing in the fetal skin
TLDR
This work has shown that in vitro cultured fetal fibroblasts produce a fibrotic-like environment and the effects of stretch on myofibroblast differentiation and reduced homing is causing the deficiency of immune cells in second-trimester fetal skin.
Prostaglandin E2 differentially modulates human fetal and adult dermal fibroblast migration and contraction: implication for wound healing
TLDR
Fetal dermal fibroblasts are partially refractory to the effects of PGE2, a major inflammatory mediator associated with dermal wound healing, which may have significant and specific relevance to the scarless fetal wound‐healing phenotype.
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