Fetal and adult human oligodendrocyte progenitor cell isolates myelinate the congenitally dysmyelinated brain

@article{Windrem2004FetalAA,
  title={Fetal and adult human oligodendrocyte progenitor cell isolates myelinate the congenitally dysmyelinated brain},
  author={Martha S. Windrem and Marta Nunes and William K. Rashbaum and Theodore H Schwartz and Robert A Goodman and Guy M. McKhann and Neeta Singh Roy and Steven A. Goldman},
  journal={Nature Medicine},
  year={2004},
  volume={10},
  pages={93-97}
}
Both late-gestation and adult human forebrain contain large numbers of oligodendrocyte progenitor cells (OPCs). These cells may be identified by their A2B5+PSA-NCAM− phenotype (positive for the early oligodendrocyte marker A2B5 and negative for the polysialylated neural cell adhesion molecule). We used dual-color fluorescence-activated cell sorting (FACS) to extract OPCs from 21- to 23-week-old fetal human forebrain, and A2B5 selection to extract these cells from adult white matter. When… 
Human fetal oligodendrocyte progenitor cells from different gestational stages exhibit substantially different potential to myelinate.
TLDR
Findings indicate that intrinsic regulatory mechanisms associated with the chronological age of the donor cells are key variables to assess when considering the myelination capacity of OPCs for cellular replacement therapy.
Human iPSC-derived oligodendrocyte progenitor cells can myelinate and rescue a mouse model of congenital hypomyelination.
Human neural progenitors from different foetal forebrain regions remyelinate the adult mouse spinal cord.
TLDR
In situ, in vitro and in vivo approaches combined to assess the oligodendrogenic potential of different human foetal forebrain regions during the first trimester of gestation reveal the great capacity of human neural progenitors to survive, extensively migrate and successfully remyelinate the spinal cord irrespective of their origin.
CD140a identifies a population of highly myelinogenic, migration-competent, and efficiently engrafting human oligodendrocyte progenitor cells
TLDR
Transplanted CD140a+ cells were highly migratory and robustly myelinated the hypomyelinated shiverer mouse brain more rapidly and efficiently than did A2B5+cells, suggesting that CD9+/CD140a- cells may constitute an even more highly enriched population of myelinogenic progenitor cells.
Derivation of oligodendrocyte precursor cells from human bone marrow stromal cells and use for re-myelination in the congenitally dysmyelinated brain
TLDR
The novel induction protocol described here provides a method for fast, simple and effective glial therapy for myelin disorders, overcoming existent hurdles of cell source restriction and time frame requirement.
Fate determination of adult human glial progenitor cells.
TLDR
Using the transcriptional profiles of acutely isolated GPCs, this work has begun to understand the operative ligand-receptor interactions involved in these processes, and has identified several key signaling pathways by which adult human GPC’s may be reliably instructed to either oligodendrocytic or astrogliotic fate.
β4 Tubulin Identifies a Primitive Cell Source for Oligodendrocytes in the Mammalian Brain
TLDR
It is proposed that βT4 cells are an endogenous cell source that can be recruited to promote neural repair in the adult telencephalon and provide in vivo support for the therapeutic potential of βT 4 cells.
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