Fetal DNA in maternal plasma: biology and diagnostic applications.

@article{Lo2000FetalDI,
  title={Fetal DNA in maternal plasma: biology and diagnostic applications.},
  author={Yuk Ming Dennis Lo},
  journal={Clinical chemistry},
  year={2000},
  volume={46 12},
  pages={
          1903-6
        }
}
  • Y. Lo
  • Published 1 December 2000
  • Medicine
  • Clinical chemistry
BACKGROUND Molecular analysis of plasma DNA during human pregnancy has led to the discovery that maternal plasma contains both fetal and maternal DNA. This valuable source of fetal DNA opens up new possibilities for noninvasive prenatal diagnosis. APPROACH Published data from the last 3 years demonstrating the feasibility and utility of analyzing fetal DNA in maternal plasma are reviewed. CONTENT The detection of fetal DNA in maternal plasma is much simpler and more robust than detecting… 
Noninvasive prenatal chromosomal aneuploidy detection using plasma cell-free nucleic acid
TLDR
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Non-invasive prenatal diagnosis (NIPD) is based on fetal DNA analysis starting from a simple peripheral blood sample, thus avoiding risks associated with conventional invasive techniques. During
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TLDR
Prenatal detection of a paternally inherited fetal aneuploidy from fetal DNA in maternal plasma is reported in an additional case involving a mother’s three consecutive pregnancies.
Epigenetic approaches for the detection of fetal DNA in maternal plasma
TLDR
An overview of the development of noninvasive prenatal diagnosis through epigenetics is presented and the basis of how fetal DNA could be detected from a large background of maternal DNA in maternal plasma based on fetal-specific DNA methylation patterns is introduced.
Methods to increase the percentage of free fetal DNA recovered from the maternal circulation.
TLDR
Addition of formaldehyde to maternal blood samples, coupled with careful processing protocols, increases the relative percentage of free fetal DNA, providing a foundation for development of noninvasive prenatal diagnostic tests to distinguish fetal DNA from maternal DNA in the maternal circulation.
Analysis of Cell-free Fetal DNA in Plasma and Serum of Pregnant Women
TLDR
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Large amounts of cell-free fetal DNA are present in amniotic fluid.
TLDR
The detection and/or quantification of fetal DNA sequences in maternal plasma have been used for a variety of clinical applications, including diagnosis of gender, Rhesus D genotype, single gene disorders, aneuploidy, and preeclampsia.
Quantitative Analysis of Male Fetal DNA in Maternal Serum of Gravid Rhesus Monkeys (Macaca mulatta)
TLDR
Data indicate that fetal male DNA is present in the maternal circulation of gravid rhesus monkeys comparable to findings in humans and further support the use of this nonhuman primate species as a model to investigate fetomaternal cell trafficking and microchimerism.
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TLDR
The present work compares single PCR on plasma DNA with nested PCR on DNA extracted from plasma or nucleated blood cells with established methodologies of nested amplification of DNA prepared from intact cells to study the release and clearance of fetal DNA from maternal plasma.
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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