Fertilizing ability of bovine spermatozoa cocultured with oviduct epithelial cells.

Abstract

The effects of bovine oviduct epithelial cell monolayers (OECM), derived from the different segments of the oviduct and under various conditions, on oocyte penetration by bovine sperm were determined by in vitro cell culture techniques. The oocyte penetration rate by sperm treated with OECM+OECM-conditioned medium was 93% (155 of 166). Sperm penetration… (More)

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