Fertilization acid release in Urechis eggs. I. The nature of the acid and the dependence of acid release and egg activation on external pH.

@article{Holland1984FertilizationAR,
  title={Fertilization acid release in Urechis eggs. I. The nature of the acid and the dependence of acid release and egg activation on external pH.},
  author={L. Holland and M. Gould-Somero and M. Paul},
  journal={Developmental biology},
  year={1984},
  volume={103 2},
  pages={
          337-42
        }
}
The amount of fertilization acid produced by eggs of Urechis caupo , monitored by automatically back-titrating egg suspensions with base, depends linearly on the pH of the seawater. Above pH 7.0, at which no acid is released (Paul, M., Dev. Biol. 43, 299-312, 1975), acid release increased approximately 0.34 pmole/egg/0.1 pH unit. Activation (germinal vesicle breakdown) depended on the amount of acid release in natural seawater; it did not occur if eggs released less than 1.5 pmole acid/egg… Expand
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