Ferrous ion-induced strand breaks in the DNA plasmid pBR322 are not mediated by hydrogen peroxide

@article{Flemmig2006FerrousIS,
  title={Ferrous ion-induced strand breaks in the DNA plasmid pBR322 are not mediated by hydrogen peroxide},
  author={J{\"o}rg Flemmig and Juergen Reinhard Arnhold},
  journal={European Biophysics Journal},
  year={2006},
  volume={36},
  pages={377-384}
}
Ferrous ion-induced generation of single and multiple strand breaks in the DNA plasmid pBR322 induces the formation of two new plasmid forms with altered electrophoretic mobility. The yield of these plasmid forms, the circular relaxed and the linear forms, depended on the applied Fe2+ concentration. This property was independent of the presence of hydrogen peroxide in the incubation mixture indicating the lack of Fenton chemistry to explain the DNA degradation. The removal of dioxygen or the… CONTINUE READING
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