The Regulatory Protein ChuP Connects Heme and Siderophore-Mediated Iron Acquisition Systems Required for Chromobacterium violaceum Virulence
It is demonstrated that ChuP connects heme and siderophore utilization by acting as a positive regulator of chuR and vbuA, which encode the TonB-dependent receptors for the uptake of heme (ChuR) and the siderophile viobactin (VbuA) in C. violaceum.
Biochemical characterization of ferric uptake regulator (Fur) from Aliivibrio salmonicida. Mapping the DNA sequence specificity through binding studies and structural modelling
The interaction between Fur from the fish pathogen Aliivibrio salmonicida (AsFur) and its target DNA is investigated using a combination of biochemical and in silico methods to gain molecular-level insight into binding specificity.
From Peptide Aptamers to Inhibitors of FUR, Bacterial Transcriptional Regulator of Iron Homeostasis and Virulence.
- BiologyACS chemical biology
The results define these novel small peptide inhibitors as lead compounds for inhibition of the FUR transcription factor with reliable structure models of two peptide-FUR complexes.
Inhibition of the ferric uptake regulator by peptides derived from anti-FUR peptide aptamers: coupled theoretical and experimental approaches.
- Biology, ChemistryACS chemical biology
The details of the interactions between peptide and protein were analyzed and a mechanism of inhibition of these anti-FUR molecules is proposed and an inhibition pocket formed by the groove between the two FUR subunits is proposed.
The Iron-Sensing Fur Regulator Controls Expression Timing and Levels of Salmonella Pathogenicity Island 2 Genes in the Course of Environmental Acidification
- BiologyInfection and Immunity
It is reported that Fur, a central regulator of iron utilization, negatively controls the expression of SPI-2 genes, a series of proteins encoded by genes within Salmonella pathogenicity island 2 (SPI-2).
Regulatory circuits in Helicobacter pylori : network motifs and regulators involved in metal-dependent responses.
- BiologyFEMS microbiology reviews
This work reviews the molecular mechanisms that underlie the activity of the regulators Fur and NikR and focuses on their involvement in the transcriptional regulatory network of the bacterium, pinpointing a surprising complexity of network motifs that interconnects them and their gene targets.
Heme-dependent metalloregulation by the iron response regulator (Irr) protein in Rhizobium and other Alpha-proteobacteria
Studies with Rhizobium reveal that the regulation of iron homeostasis in bacteria is more diverse than has been generally assumed.
Functional characterization of the dimerization domain of the ferric uptake regulator (Fur) of Pseudomonas aeruginosa.
- BiologyThe Biochemical journal
The recombinant C-terminal dimerization domain of the Pseudomonas aeruginosa Fur (ferric uptake regulator) protein expressed in and purified from Escherichia coli is evaluated to conclude that the domain is a reasonable model for many properties of the full-length protein and is amenable to some analyses that the limited solubility of theFull-length Protein prevents.