Ferric iron uptake in Erwinia chrysanthemi mediated by chrysobactin and related catechol-type compounds

@article{Persmark1992FerricIU,
  title={Ferric iron uptake in Erwinia chrysanthemi mediated by chrysobactin and related catechol-type compounds},
  author={Magnus Persmark and Dominique Expert and John B. Neilands},
  journal={Journal of Bacteriology},
  year={1992},
  volume={174},
  pages={4783 - 4789}
}
Erwinia chrysanthemi 3937 possesses a saturable, high-affinity transport system for the ferric complex of its native siderophore chrysobactin, [N-alpha-(2,3-dihydroxybenzoyl)-D-lysyl-L-serine]. Uptake of 55Fe-labeled chrysobactin was completely inhibited by respiratory poison or low temperature and was significantly reduced in rich medium. The kinetics of chrysobactin-mediated iron transport were determined to have apparent Km and Vmax values of about 30 nM and of 90 pmol/mg.min, respectively… 
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Chrysobactin-dependent iron acquisition in Erwinia chrysanthemi. Functional study of a homolog of the Escherichia coli ferric enterobactin esterase.
TLDR
Mössbauer spectroscopy of whole cells at various states of (57)Fe-labeled chrysobactin uptake showed that a functional cbsH gene is not required for iron removal from ferric enterobactin in E. chrysanthemi, and the CbsH protein may therefore be regarded as a peptidase that prevents the bacterial cells from being intracellularly iron-depleted by chry SoBactin.
Synthesis of optically pure chrysobactin and immunoassay development
TLDR
A monoclonal antibody to ferric chrysobactin was developed and characterized as IgM and this immunoassay may provide a useful method for the detection of chry sobactsin in plant samples.
Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore
TLDR
Results suggest an uptake system in which only the synthesis of the siderophore is regulated by the iron available for growth, and the release of 2,3-DHBA under iron starvation was not associated with the expression of new outer membrane proteins.
Production of 2,3-dihydroxybenzoic acid by Brucella species
TLDR
No clear correlation between catechol production and growth under iron-limiting conditions was observed and, quantitatively, production of catechols varied widely among the different strains with no relationship to species, biotypes, or serotypes of Brucella.
Iron and plant pathogenesis: the systemic soft rot disease induced by Erwinia chrysanthemi 3937 on saintpaulia plants
TLDR
A molecular study of the chrysobactin system with a special emphasis on its regulation by iron found it to modulate the expression of several pectinase-encoding genes of major importance for the virulence of microbial siderophores.
Regulation of pectinolysis in Erwinia chrysanthemi.
TLDR
The regulation of pel genes requires several regulatory systems, including the KdgR repressor, which mediates the induction of all the pectinolysis genes in the presence of pectin catabolites.
FvtA Is the Receptor for the Siderophore Vanchrobactin in Vibrio anguillarum: Utility as a Route of Entry for Vanchrobactin Analogues
TLDR
This study characterized two genes, fvtA and orf13, encoding potential TonB-dependent ferric-vanchrobactin receptors in serotype O2 V. anguillarum strain RV22 and found that FvtA is the route of entry for vanch Robactin analogues, and there is evidence that it recognizes primarily the catechol-iron center.
Two Genomic Regions Involved in Catechol Siderophore Production by Erwinia carotovora
Two regions involved in catechol biosynthesis (cbs) of Erwinia carotovora W3C105 were cloned by functional complementation of Escherichia coli mutants that were deficient in the biosynthesis of the…
Comparison of the main siderophores produced by some species of Streptomyces
TLDR
The uptake rates of 55Fe-labeled ferrioxamines by S. lividans and S. viridosporus showed that the G form was incorporated less efficiently than the B and E forms, which was the main siderophore of S. ambofaciens.
Erwinia, a Plant Pathogen
TLDR
It has been shown that Erwinia chrysanthemi 3937 induces systemic symptoms in the plant model Arabidopsis thaliana, and a pathosystem should help to identify plant iron mobilization reactions that may occur during pathogenesis.
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TLDR
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