Fermentative production of tryptophan by a stable recombinant strain of Corynebacterium glutamicum with a modified serine-biosynthetic pathway.

@article{Ikeda1994FermentativePO,
  title={Fermentative production of tryptophan by a stable recombinant strain of Corynebacterium glutamicum with a modified serine-biosynthetic pathway.},
  author={Masato Ikeda and Kazuhiro Nakanishi and Kosuke Kino and Ryoichi Katsumata},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1994},
  volume={58 4},
  pages={674-8}
}
Introduction of plasmid pKW99, which coexpresses the deregulated 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase and tryptophan-biosynthetic enzymes, into tryptophan-producing Corynebacterium glutamicum KY10894 resulted in a marked increase (54%) in yield of tryptophan production (43 g/liter), but incurred two problems. One was a decline in sugar consumption at the late stage of fermentation, and the other the loss of the plasmid in the absence of selective pressure. The retarded sugar… CONTINUE READING