Fermentation of 10% (w/v) Sugar to D(−)-Lactate by Engineered Escherichia coli B

@article{Zhou2005FermentationO1,
  title={Fermentation of 10\% (w/v) Sugar to D(−)-Lactate by Engineered 
Escherichia coli B},
  author={S. Zhou and Lorraine P. Yomano and Keelnatham T Shanmugam and Lonnie O'Neal Ingram},
  journal={Biotechnology Letters},
  year={2005},
  volume={27},
  pages={1891-1896}
}
Derivatives of ethanologenic Escherichia coli K011 were constructed for d(−)-lactate production by deleting genes encoding competing pathways followed by metabolic evolution, a growth-based selection for mutants with improved performance. Resulting strains, SZ132 and SZ186, contain native genes for sucrose utilization. No foreign genes are present in SZ186. Strain SZ132 also contains a chromosomally integrated endoglucanase gene (Erwinia chrysanthemi celY). Strain SZ132 produced over 1 mol… Expand
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