Femtosecond transient absorption study on relaxation intermediates in oxymyoglobin.

  title={Femtosecond transient absorption study on relaxation intermediates in oxymyoglobin.},
  author={Shoji Ishizaka and Teruhide Wada and Noboru Kitamura},
  journal={Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology},
  volume={8 4},
We conducted femtosecond transient absorption spectroscopy of oxymyoglobin (MbO(2)) in the wavelength region of 450-750 nm to observe the charge transfer (CT) state of the heme in MbO(2). The transient absorption spectrum just after photoexcitation (t = 0-0.25 ps) was assigned to superposition of S(1)-S(n) absorption (450-750 nm) and ground-state bleaching (540 and 580 nm) of MbO(2). At t > 0.25 ps, a new absorption band appeared at around 680 nm with the decay time of approximately 5 ps. This… CONTINUE READING