Femtosecond spectroscopic observations of initial intermediates in the photocycle of the photoactive yellow protein from Ectothiorhodospira halophila.

@article{Devanathan1999FemtosecondSO,
  title={Femtosecond spectroscopic observations of initial intermediates in the photocycle of the photoactive yellow protein from Ectothiorhodospira halophila.},
  author={Savitha Devanathan and Andr{\'e}s Pacheco and L. Ujj and Michael A. Cusanovich and Gordon Tollin and S Lin and Neal W. Woodbury},
  journal={Biophysical journal},
  year={1999},
  volume={77 2},
  pages={1017-23}
}
Femtosecond time-resolved absorbance measurements were used to probe the subpicosecond primary events of the photoactive yellow protein (PYP), a 14-kD soluble photoreceptor from Ectothiorhodospira halophila. Previous picosecond absorption studies from our laboratory have revealed the presence of two new early photochemical intermediates in the PYP photocycle, I(0), which appears in </=3 ps, and I(0)(double dagger), which is formed in 220 ps, as well as stimulated emission from the PYP excited… CONTINUE READING

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