Femtosecond infrared laser-an efficient and safe in vivo gene delivery system for prolonged expression.

@article{Zeira2003FemtosecondIL,
  title={Femtosecond infrared laser-an efficient and safe in vivo gene delivery system for prolonged expression.},
  author={E. Zeira and A. Manevitch and A. Khatchatouriants and O. Pappo and E. Hyam and M. Darash-Yahana and E. Tavor and A. Honigman and A. Lewis and E. Galun},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2003},
  volume={8 2},
  pages={
          342-50
        }
}
  • E. Zeira, A. Manevitch, +7 authors E. Galun
  • Published 2003
  • Biology, Medicine
  • Molecular therapy : the journal of the American Society of Gene Therapy
The major advantages of "naked DNA gene therapy" are its simplicity and a low or negligible immune response. Gene delivery by DNA electroporation (EP) involves injection of DNA and the application of a brief electric pulse to enhance cellular permeability. Although EP is an efficient gene transduction technique in rodents, it requires much higher voltages (>500 V) in larger animals, and hence, in practice it would be hazardous for human patients, as it would cause serious tissue damage. To… Expand
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An overview of the theory and practice of electroporative gene transfer is provided to aid researchers that wish to employ the method for preclinical and translational gene therapy, NAV, and functional genomic research. Expand
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A variety of mammalian cells can be directly transfected with DNA without perturbing their structure by first creating a tiny, localized perforation in the membrane using ultrashort (femtosecond), high-intensity, near-infrared laser pulses. Expand
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  • Biology, Medicine
  • Molecular therapy : the journal of the American Society of Gene Therapy
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The present study shows that the intraarterial delivery of naked plasmid DNA into the femoral artery of rats can also enable high levels of foreign gene expression throughout the limb skeletal muscles in rhesus monkeys, and suggests that the procedure is likely to enable efficient and stable gene expression in human muscle without substantial toxicity. Expand
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