Female-Associated DNA Polymorphisms of Hemp (Cannabis sativa L.)

  title={Female-Associated DNA Polymorphisms of Hemp (Cannabis sativa L.)},
  author={Hong Shao and Shu-juan Song and R. Clarke},
  journal={Journal of Industrial Hemp},
  pages={5 - 9}
Abstract The sex identification of pre-floral hemp (Cannabis sativa L.) is problematic for taxonomists and plant breeders, because the distinguishing morphological characteristics of male and female plants do not appear before flowering. Employing the RAPD (Random Amplified Polymorphic DNA) technique with 15 random primers, we have found that the genomes of female individuals bear female-associated polymorphic DNA fragments (870 bp and 1160 bp amplified by primer OPA-04; 1680 bp by primer OPF… Expand
Dioecious hemp (Cannabis sativa L.) plants do not express significant sexually dimorphic morphology in the seedling stage
Some economically important crop species are dioecious, producing pollen and ovules on distinct, unisexual, individuals. On-the-spot diagnosis of sex is important to breeders and farmers for cropExpand
Genomics and Molecular Markers in Cannabis sativa L.
In this chapter, the development of different types of molecular markers and their application to the most relevant traits for Cannabis breeding are described and discussed. Expand
Potential of marker-assisted selection in hemp genetic improvement
Active research is carried out by several groups in the field of identification and characterization of the genes involved in fiber quality and quantity, and in the determination of monoecy, another very important target of hemp breeding. Expand
Development and validation of genetic markers for sex and cannabinoid chemotype in Cannabis sativa L.
High‐throughput PACE (PCR Allele Competitive Extension) assays for C. sativa plant sex and cannabinoid chemotype revealed segregation in hemp populations, and resolved plants producing predominantly THC, predominantly CBD, and roughly equal amounts of THC and CBD. Expand
Evolution and Classification of Cannabis sativa (Marijuana, Hemp) in Relation to Human Utilization
This review examines the evolving ways Cannabis has been used from ancient times to the present, and how human selection has altered the morphology, chemistry, distribution and ecology of domesticated forms by comparison with related wild plants. Expand
Genetic Diversity and Population Structure of Cannabis Based on the Genome-Wide Development of Simple Sequence Repeat Markers
  • Jiangjiang Zhang, Jiangtao Yan, +11 authors Defang Li
  • Medicine, Biology
  • Frontiers in Genetics
  • 2020
The Cannabis population did not show mutation-drift equilibrium following analysis via the infinite allele model, and these results provide guidance for the molecular breeding and further investigation of Cannabis. Expand
Recent advances in cannabis biotechnology
The current manuscript reviews the advances in Cannabis biotechnology, including molecular markers, microRNA, omics-based methods, and functional genes related to the terpene and cannabinoid biosynthesis as well as fiber quality. Expand
Cannabis and Natural Cannabis Medicines
This chapter briefly explains the life cycle, origin, early evolution, and domestication of Cannabis, plus provides a brief history of drug Cannabis breeding and looks into the future of Cannabis as a source of medicines. Expand
Development of a Comprehensive Genetic Tool for Identification of Cannabis sativa Samples for Forensic and Intelligence Purposes
This resource was prepared by the author(s) using Federal funds provided by the U.S. Department of Justice and does not necessarily reflect the official position or policies of the United States Department of justice. Expand


Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)
A 400-bp RAPD marker generated by a primer of random decamer sequence has been found associated with the male sex phenotype in 14 dioecious cultivars and accessions of hemp and is suitable for a precise, early and rapid identification of male plants during breeding programs of dIOecious and monoecious hemp. Expand
A male-associated DNA sequence in a dioecious plant, Cannabis sativa L.
Male-associated DNA sequences were analyzed in a dioecious plant, Cannabis sativa L. (family: Moraceae), which is known to have sex chromosomes and exhibited no significant similarity to previously reported sequences. Expand
An Effective Procedure for the RAPD Analysis of Hemp Cannabis sativa L.
Using Cannabis herbarium specimens as a source of genetic materials, the correlative conditions of the Polymerase chain reaction (PCR), and Random amplified polymorphic DNA (RAPD) were examined separately, an effective procedure for the RAPD analysis of Cannabis was obtained. Expand
Genetic diversity of Cannabis sativa germplasm based on RAPD markers
RAPD markers provide a powerful tool for the investigation of genetic variation in cultivars/accessions of hemp and Variability within the two cultivars ‘Carmagnola’ and ‘Fibranova1’ was shown, suggesting good possibilities for long–term selection work. Expand
Ribosomal DNA analysis as a tool for the identification of Cannabis sativa L. specimens of forensic interest.
Hemp ITS2 varied very little among cultivars, but was consistently different from that of hops, which belongs to the only other genus of family Cannabinaceae. Expand
Identification of Cannabis sativa L. (Cannabaceae) using restriction profiles of the Internal Transcribed Spacer II (ITS2).
  • G. S. Gigliano
  • Medicine, Biology
  • Science & justice : journal of the Forensic Science Society
  • 1998
The paper describes the construction of a restriction profile of the Internal Transcribed Spacer II (ITS2) of Cannabis sativa L. Nuclear Ribosomal DNA, using digestion by a selected number ofExpand
Preliminary data on the usefulness of internal transcribed spacer I (ITS1) sequence in Cannabis sativa L. identification.
Preliminary results show that the ITS1 is an ideal molecule for the identification of a sample suspected to be marijuana. Expand
Detection and identification of cannabis by DNA.
A method for the identification of cannabis using DNA-specific primers has been developed and is described here and identifies the presence of Cannabis sativa. Expand
AFLP-marker for male plants of hemp (Cannabis sativa L.)
  • Poster presented at the 3rd Bioresource Hemp Symposium,
  • 2000
DNA markers associated with sex phenotype in hemp (Cannabis sativa L.)
  • Proceedings of the Bast Fibrous Plants–Today and Tomorrow. Russia
  • 1998