Feline coronavirus quantitative reverse transcriptase polymerase chain reaction on effusion samples in cats with and without feline infectious peritonitis

@article{Longstaff2017FelineCQ,
  title={Feline coronavirus quantitative reverse transcriptase polymerase chain reaction on effusion samples in cats with and without feline infectious peritonitis},
  author={Louise Longstaff and Emily Porter and V J Crossley and S. Hayhow and Christopher R. Helps and S{\'e}verine Tasker},
  journal={Journal of Feline Medicine and Surgery},
  year={2017},
  volume={19},
  pages={240 - 245}
}
Objectives The aim of the study was to determine whether feline coronavirus (FCoV) RNA in effusion samples can be used as a diagnostic marker of feline infectious peritonitis (FIP); and in FCoV RNA-positive samples to examine amino acid codons in the FCoV spike protein at positions 1058 and 1060 where leucine and alanine, respectively, have been associated with systemic or virulent (FIP) FCoV infection. Methods Total RNA was extracted from effusion samples from 20 cats with confirmed FIP and 23… 

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