Feline coronavirus quantitative reverse transcriptase polymerase chain reaction on effusion samples in cats with and without feline infectious peritonitis

@article{Longstaff2017FelineCQ,
  title={Feline coronavirus quantitative reverse transcriptase polymerase chain reaction on effusion samples in cats with and without feline infectious peritonitis},
  author={Louise Longstaff and Emily Porter and V J Crossley and S. Hayhow and Christopher R. Helps and S{\'e}verine Tasker},
  journal={Journal of Feline Medicine and Surgery},
  year={2017},
  volume={19},
  pages={240 - 245}
}
Objectives The aim of the study was to determine whether feline coronavirus (FCoV) RNA in effusion samples can be used as a diagnostic marker of feline infectious peritonitis (FIP); and in FCoV RNA-positive samples to examine amino acid codons in the FCoV spike protein at positions 1058 and 1060 where leucine and alanine, respectively, have been associated with systemic or virulent (FIP) FCoV infection. Methods Total RNA was extracted from effusion samples from 20 cats with confirmed FIP and 23… Expand
Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis
TLDR
It is demonstrated that S gene mutation analysis in FCoVs does not substantially improve the ability to diagnose FIP as compared to detection of FCoV alone. Expand
Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus mutations in effusion and serum/plasma of cats to diagnose feline infectious peritonitis
TLDR
Although specificity of the test in effusions was high, one false positive result occurred, and the use of serum/plasma cannot be recommended due to a low viral load in blood. Expand
Discrepancies between feline coronavirus antibody and nucleic acid detection in effusions of cats with suspected feline infectious peritonitis
TLDR
Combining the two methods, i.e., antibody and RNA detection would help improving the intra-vitam diagnosis of effusive FIP. Expand
Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis
TLDR
In these 20 cats with FIP, FCoVs with S gene mutations were found in every cat in at least one tissue or fluid sample, which highlights the association between mutated S gene and systemic FCoV spread. Expand
Diagnosis of non-effusive feline infectious peritonitis by reverse transcriptase quantitative PCR from mesenteric lymph node fine-needle aspirates
TLDR
In cases with a high index of suspicion of disease, RT-qPCR targeting FCoV in MLN FNA can provide important information to support the ante-mortem diagnosis of non-effusive FIP. Expand
Reverse transcriptase loop-mediated isothermal amplification for the detection of feline coronavirus
TLDR
A reverse transcription loop mediated isothermal amplification (RT-LAMP) assay seems optimal to confirm the presence of the virus, but not applicable to exclude it. Expand
Comparison of the performance of laboratory tests in the diagnosis of feline infectious peritonitis
We compared the performance of clinicopathologic and molecular tests used in the antemortem diagnosis of feline infectious peritonitis (FIP). From 16 FIP and 14 non-FIP cats, we evaluatedExpand
Oral Mutian®X stopped faecal feline coronavirus shedding by naturally infected cats
TLDR
The first report of the successful elimination of faecal FCoV shedding in chronically infected cats is reported, and Mutian X given orally cleared the virus. Expand
Utility of an immunocytochemical assay using aqueous humor in the diagnosis of feline infectious peritonitis
TLDR
The diagnostic utility of an immunocytochemical assay in aqueous humor of cats suspected of having FIP is limited and false‐positive results occurred and specificity is most important in the diagnosis of FIP. Expand
Diagnosis of feline infectious peritonitis: Update on evidence supporting available tests
  • S. Tasker
  • Medicine
  • Journal of feline medicine and surgery
  • 2018
TLDR
This review provides a comprehensive overview of how to approach the diagnosis of FIP, focusing on the tests available to the veterinary practitioner and recently published evidence supporting their use. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 25 REFERENCES
Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitis
TLDR
The methionine to leucine substitution at position 1058 in the FCoV spike protein is indicative of systemic spread of F CoV from the intestine, rather than a virus with the potential to cause FIP. Expand
The detection of feline coronaviruses in blood samples from cats by mRNA RT-PCR
TLDR
It was observed that the positivity rate was highest in cats aged between 6 months–1 year old, and the findings suggest that FCoVs may be present in the blood samples from healthy cats as well as cats with clinical FIP. Expand
Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis
TLDR
The co-cultivation system proved to be a suitable method for the culture of field strains of FCoV from blood samples, so long as the cultures were maintained for at least 4 weeks, and viraemia was detected as frequently as by RT-PCR on RNA extracted directly from peripheral blood mononuclear cells. Expand
Detection of feline coronavirus in cerebrospinal fluid for diagnosis of feline infectious peritonitis in cats with and without neurological signs
TLDR
This real-time RT-PCR detecting FCoV RNA in CSF can be considered a reliable specific tool for the diagnosis of FIP, especially if performed using serum or plasma. Expand
Evaluation of real-time RT-PCR for the quantification of FCoV shedding in the faeces of domestic cats
TLDR
Although real-time RT-PCR provides an excellent method for detecting the presence of viral shedding, quantification of FCoV RNA in faecal material has to take into account the possible effects of RT- PCR inhibitors. Expand
Detection of Ascitic Feline Coronavirus RNA from Cats with Clinically Suspected Feline Infectious Peritonitis
TLDR
Ascitic feline coronavirus RNA was examined in 854 cats with suspected feline infectious peritonitis (FIP) by RT-PCR, indicating that FIP more frequently develops in males among FCoV-infected cats. Expand
Levels of feline infectious peritonitis virus in blood, effusions, and various tissues and the role of lymphopenia in disease outcome following experimental infection
TLDR
The effect of lymphopenia on disease outcome was reconfirmed and FIPV genomic RNA was found to be highly macrophage associated within diseased tissues and effusions as determined by RT-qPCR and immunohistochemistry but was not present in blood. Expand
Diagnostic utility of a direct immunofluorescence test to detect feline coronavirus antigen in macrophages in effusive feline infectious peritonitis
TLDR
Direct immunofluorescence testing to detect feline coronavirus in macrophages in effusion specimens has been reported to have 100% specificity and has been recommended as an antemortem confirmatory test. Expand
In Vivo Diagnosis of Feline Infectious Peritonitis by Comparison of Protein Content, Cytology, and Direct Immunofluorescence Test on Peritoneal and Pleural Effusions
  • S. Paltrinieri, M. Parodi, G. Cammarata
  • Medicine
  • Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
  • 1999
In this study, the results obtained using 3 different diagnostic methods (anti-FCoV direct immunofluorescence test, cytology, and protein analysis) for 110 cats with effusions over a 5-year periodExpand
Comparison of Different Tests to Diagnose Feline Infectious Peritonitis
TLDR
Positive and negative predictive values of Rivalta's test, a test that distinguishes between exudates and transudates, and immunofluorescence staining of coronavirus antigen in macrophages were investigated, and diagnostic assays on the fluid in cats with body effusion had good predictive values. Expand
...
1
2
3
...