Feeder-independent culture of human embryonic stem cells

@article{Ludwig2006FeederindependentCO,
  title={Feeder-independent culture of human embryonic stem cells},
  author={Tenneille E. Ludwig and V. Christina B. Bergendahl and Mark E Levenstein and Junying Yu and Mitchell D. Probasco and James A. Thomson},
  journal={Nature Methods},
  year={2006},
  volume={3},
  pages={637-646}
}
We recently reported the development of TeSR1, a serum-free, animal product–free medium that supports the derivation and long-term feeder-independent culture of human embryonic stem cells. Although the derivation of new human embryonic stem cell lines in those defined conditions offered an important proof of principle, the costs of some of the defined components in that culture system made it impractical for everyday research use. Here we describe modifications to the medium (mTeSR1) that… CONTINUE READING
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