Feedback phase correction of Bessel beams in confocal line light-sheet microscopy: a simulation study.
@article{Moosavi2013FeedbackPC,
title={Feedback phase correction of Bessel beams in confocal line light-sheet microscopy: a simulation study.},
author={S. Hoda Moosavi and Cristian Gohn-Kreuz and Alexander Rohrbach},
journal={Applied optics},
year={2013},
volume={52 23},
pages={
5835-42
}
}Confocal line detection has been shown to improve contrast in light-sheet-based microscopy especially when illuminating the sample by Bessel beams. Besides their self-reconstructing capability, the stability in propagation direction of Bessel beams allows to block the unwanted emission light from the Bessel beam's ring system. However, due to phase aberrations induced especially at the border of the specimen, Bessel beams may not propagate along lines parallel to the slit detector. Here we…
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References
SHOWING 1-10 OF 21 REFERENCES
A line scanned light-sheet microscope with phase shaped self-reconstructing beams.
- PhysicsOptics express
- 2010
This work presents an add-on module to a standard inverted microscope using a scanned beam that is shaped in phase and amplitude by a spatial light modulator to increase both image quality and penetration depth of illumination beams in strongly scattering media.
Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media.
- PhysicsNature communications
- 2012
The beam's propagation stability along one straight line enables the use of a confocal line principle, resulting in a significant increase in image contrast, while the axial resolution could be improved by nearly 100% relative to the standard light-sheet techniques using scanned Gaussian beams.
Self-reconstructing sectioned Bessel beams offer submicron optical sectioning for large fields of view in light-sheet microscopy.
- PhysicsOptics express
- 2013
Sectioned Bessel beams are introduced and it is demonstrated that these beams exhibit self-reconstruction capabilities and penetration depths into thick scattering media equal to those of conventional Bessel beam.
Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation.
- PhysicsOptics express
- 2013
The results reveal that light-sheets generated by pulsed near-infrared Bessel beams and two photon excitation provide the best image resolution, contrast at both a minimum amount of artifacts and signal degradation along the propagation of the beam into the sample.
Scanned light sheet microscopy with confocal slit detection.
- PhysicsOptics express
- 2012
The imaging principle presented here merges the benefits of scanned light sheet microscopy and line-scanning confocal imaging, thereby enhancing contrast, optical sectioning and signal-to-noise ratio.
Artifacts resulting from imaging in scattering media: a theoretical prediction.
- PhysicsOptics letters
- 2009
A theoretical perspective of how to extend the point-spread function arithmetic from ideal imaging to realistic imaging including ghost images, which are generated by scattered light and are low-correlated with the ideal image.
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
- BiologyNature Methods
- 2011
Scanned Bessel beams are used in conjunction with structured illumination and/or two-photon excitation to create thinner light sheets better suited to three-dimensional (3D) subcellular imaging.
Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
- BiologyScience
- 2004
Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination…
Light propagation in graded-index optical fibers.
- PhysicsApplied optics
- 1978
An accurate numerical method is described for solving the Helmholtz equation for a general class of optical fibers. The method yields detailed information about the spatial and angular properties of…
Microscopy with self-reconstructing beams
- Physics
- 2010
A prototype microscope built with self-reconstructing Bessel beams is shown to be able to reduce scattering artifacts as well as increase image quality and penetration depth in three-dimensional…










