Fedbatch culture and dynamic nutrient feeding.

  title={Fedbatch culture and dynamic nutrient feeding.},
  author={Katie Fraass Wlaschin and Wei-Shou Hu},
  journal={Advances in biochemical engineering/biotechnology},
In the past decade, we have seen a rapid expansion in mammalian cell based therapeutic proteins reaching clinical applications. This increased demand has been met with much increased productivity through intensive process development. During this time, fedbatch culture processes have emerged as the predominant mode for producing recombinant proteins. In this review, we discuss the fundamentals of fedbatch culture process design, focusing on the use of stoichiometric nutrient requirements for… CONTINUE READING