Fed-batch culture of insect cells: a method to increase the yield of recombinant human nerve growth factor (rhNGF) in the baculovirus expression system.

@article{Nguyen1993FedbatchCO,
  title={Fed-batch culture of insect cells: a method to increase the yield of recombinant human nerve growth factor (rhNGF) in the baculovirus expression system.},
  author={B Nguyen and Kurt Jarnagin and Steven Williams and Helen Chan and James W Barnett},
  journal={Journal of biotechnology},
  year={1993},
  volume={31 2},
  pages={205-17}
}
A fed-batch method was developed which increased the density of insect cells (Spodoptera frugiperda, Sf-9 cells) in suspension culture and the feeding of nutrients improved the yield of a recombinant protein produced by a baculovirus expression system. Analysis of spent medium samples indicated that depletions of glucose and glutamine correlated with the retardation of cell growth. Feeding of a mixture of nutrients consisting of glucose, glutamine, yeastolate and lipids solution restored the… CONTINUE READING
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