Fed-batch culture of Sf-9 cells supports 3 3 10 7 cells per ml and improves baculovirus-expressed recombinant protein yields

Abstract

Yields of cell and baculovirus-expressed recombinant protein in Sf-9 cultures were improved using fed-batch addition of glucose, amino acids, lipids, yeastolate ultrafiltrate, vitamins, iron and trace metals. Using this approach, the highest maximum cell and recombinant b-galactosidase volumetric yields obtained, 3 3 10 .ml and 900 units.ml , respectively… (More)
DOI: 10.1023/A:1018378529299

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