Fecal microbiota analysis: an overview of sample collection methods and sequencing strategies.

@article{Thomas2015FecalMA,
  title={Fecal microbiota analysis: an overview of sample collection methods and sequencing strategies.},
  author={Vincent Thomas and James Clark and Jo{\"e}l Dore},
  journal={Future microbiology},
  year={2015},
  volume={10 9},
  pages={1485-504}
}
Despite huge interest, there are still no universally accepted standards to conduct clinical studies in the field of gut microbiota analysis. Stool material is frequently used as a proxy of gut microbiota, but many different protocols can be used for collection and DNA extraction. Whereas 16S rRNA encoding gene amplification and sequencing has been widely used to study the composition of bacterial populations, it is now being challenged by the random, shotgun approach that brings far more… CONTINUE READING

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